Bacteria can be grown in lab by providing adequate nutrients. pH, temperature, aeration, salt concentrations and adjusting the ionic strength of the medium. This is made possible using culture media.
Substances such as blood, serum, egg are added to provide nutritional support. They are used to grow bacteria which are more exacting in their nutritional needs.
Eg. Blood Agar, Chocolate Agar and egg media
Substances which have a stimulating effect on the bacteria to be grown or an inhibitory effect on those to be supressed are incorporated on a liquid media.
Eg. Tertathiionate broth, Selenite F broth, Thioglycolate, monsur’s taurocholate tellurite, peptone water, Alkaline peptone water.
Substances which have a stimulating effect on the bacteria to be grown or an inhibitory effect on those to be supressed are incorporated in the solid medium.
Eg. Deosycholate citrate agar, Lowenstein jensen medium.
Media contains an indicator which changes colour when a bacteria grows in them.
Eg. Wilson and blair medium, Mcleod’d Medium
A medium has substances which bring out different characteristics of the bacteria thus helps to distinguish between them.
Eg.MacConkey’s medium, CLED medium
You Might Have Missed These Posts